coding sequence (cds) region of the goat slam gene Search Results


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Thermo Fisher gene exp ace2 hs01085333 m1
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Vector Laboratories normal goat serum
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Santa Cruz Biotechnology goat anti cart

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Jackson Immuno goat antimouse antibody conjugated to cy5

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Jackson Immuno gold conjugated antibody

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Vector Laboratories biotinylated goat anti rabbit igg

Biotinylated Goat Anti Rabbit Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GE Healthcare anti gst
( A ) Binding assay in a cell culture system. The 293 cells were transiently transfected <t>with</t> <t>FAM20A-Flag</t> (lane 2) and FAM20C-HA (lanes 2 and 3). Cell lysates were prepared and immunoprecipitated (IP) with anti-Flag antibody. The interaction was detected by Western blotting (WB) with anti-HA antibody (upper panel). Presence of FAM20C-HA (middle panel) and FAM20A-Flag (lower panel) in the same lysates was verified. ( B ) FAM20A-FAM20C interaction is independent from FAM20C kinase activity. The 293 cells were transfected with FAM20A WT-HA and FAM20C WT-V5 (WT), FAM20C D478A-V5 (DA), or FAM20C P328S-V5 (PS). Cell lysates were immunoprecipitated with anti-V5 antibody and the interaction was detected by WB analysis with anti-HA antibody (upper panel). Presence of FAM20A WT-HA (middle panel) and FAM20C forms (lower panel) in the same lysates was confirmed. ( C ) Binding assay by <t>GST</t> pull down. The rhFAM20C WT-V5/His or rhFAM20C D478A-V5/His protein was incubated with either GST protein alone or GST-FAM20A protein and coupled to glutathione beads. After washing the beads, the bound proteins were visualized by WB analysis with anti-V5 antibody (upper panel). Presence of rhFAM20C WT-V5/His (upper panel, lane 5), rhFAM20C D478A-V5/His (upper panel, lane 6), GST and GST-FAM20A (lower panels) proteins were assessed by WB analysis.
Anti Gst, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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90
GenScript corporation hrp-labeled goat anti-mouse igg
( A ) Binding assay in a cell culture system. The 293 cells were transiently transfected <t>with</t> <t>FAM20A-Flag</t> (lane 2) and FAM20C-HA (lanes 2 and 3). Cell lysates were prepared and immunoprecipitated (IP) with anti-Flag antibody. The interaction was detected by Western blotting (WB) with anti-HA antibody (upper panel). Presence of FAM20C-HA (middle panel) and FAM20A-Flag (lower panel) in the same lysates was verified. ( B ) FAM20A-FAM20C interaction is independent from FAM20C kinase activity. The 293 cells were transfected with FAM20A WT-HA and FAM20C WT-V5 (WT), FAM20C D478A-V5 (DA), or FAM20C P328S-V5 (PS). Cell lysates were immunoprecipitated with anti-V5 antibody and the interaction was detected by WB analysis with anti-HA antibody (upper panel). Presence of FAM20A WT-HA (middle panel) and FAM20C forms (lower panel) in the same lysates was confirmed. ( C ) Binding assay by <t>GST</t> pull down. The rhFAM20C WT-V5/His or rhFAM20C D478A-V5/His protein was incubated with either GST protein alone or GST-FAM20A protein and coupled to glutathione beads. After washing the beads, the bound proteins were visualized by WB analysis with anti-V5 antibody (upper panel). Presence of rhFAM20C WT-V5/His (upper panel, lane 5), rhFAM20C D478A-V5/His (upper panel, lane 6), GST and GST-FAM20A (lower panels) proteins were assessed by WB analysis.
Hrp Labeled Goat Anti Mouse Igg, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/coding+sequence+%28cds%29+region+of+the+goat+slam+gene/10__1016_slash_j__aquaculture__2014__04__023-78-15-18?v=GenScript+corporation
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BID Antibody raised in Goat validated in E, WB, IF in Human.
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N/A
Goat polyclonal to CDSN. Isotype Note: IgG Host Note: Goat Conjugation Note: Unconjugated Reactivity Note: Human Application Note: ELISA, WB
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Anti-p33 ING1 (GOAT) Antibody - 600-101-282
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Image Search Results


Journal: iScience

Article Title: GPR162 is a beta cell CART receptor

doi: 10.1016/j.isci.2023.108416

Figure Lengend Snippet:

Article Snippet: The following antibody combinations were used for the PLA: goat anti-GPR162 (sc-138323, dilution 1:500, Santa Cruz) with rabbit anti-CART (code 2059A, dilution 1:5000, kindly provided by Dr. Jes T. Clausen, Novo Nordisk, Måløv, Denmark), and goat anti-CART (code sc-18068, dilution 1:400, Santa Cruz) with rabbit anti-GPR162 (code PA5-33655, dilution 1:500, Thermo Scientific).

Techniques: Isolation, Recombinant, RNA Extraction, Enzyme-linked Immunosorbent Assay, RNA Sequencing, Software

( A ) Binding assay in a cell culture system. The 293 cells were transiently transfected with FAM20A-Flag (lane 2) and FAM20C-HA (lanes 2 and 3). Cell lysates were prepared and immunoprecipitated (IP) with anti-Flag antibody. The interaction was detected by Western blotting (WB) with anti-HA antibody (upper panel). Presence of FAM20C-HA (middle panel) and FAM20A-Flag (lower panel) in the same lysates was verified. ( B ) FAM20A-FAM20C interaction is independent from FAM20C kinase activity. The 293 cells were transfected with FAM20A WT-HA and FAM20C WT-V5 (WT), FAM20C D478A-V5 (DA), or FAM20C P328S-V5 (PS). Cell lysates were immunoprecipitated with anti-V5 antibody and the interaction was detected by WB analysis with anti-HA antibody (upper panel). Presence of FAM20A WT-HA (middle panel) and FAM20C forms (lower panel) in the same lysates was confirmed. ( C ) Binding assay by GST pull down. The rhFAM20C WT-V5/His or rhFAM20C D478A-V5/His protein was incubated with either GST protein alone or GST-FAM20A protein and coupled to glutathione beads. After washing the beads, the bound proteins were visualized by WB analysis with anti-V5 antibody (upper panel). Presence of rhFAM20C WT-V5/His (upper panel, lane 5), rhFAM20C D478A-V5/His (upper panel, lane 6), GST and GST-FAM20A (lower panels) proteins were assessed by WB analysis.

Journal: Scientific Reports

Article Title: FAM20A binds to and regulates FAM20C localization

doi: 10.1038/srep27784

Figure Lengend Snippet: ( A ) Binding assay in a cell culture system. The 293 cells were transiently transfected with FAM20A-Flag (lane 2) and FAM20C-HA (lanes 2 and 3). Cell lysates were prepared and immunoprecipitated (IP) with anti-Flag antibody. The interaction was detected by Western blotting (WB) with anti-HA antibody (upper panel). Presence of FAM20C-HA (middle panel) and FAM20A-Flag (lower panel) in the same lysates was verified. ( B ) FAM20A-FAM20C interaction is independent from FAM20C kinase activity. The 293 cells were transfected with FAM20A WT-HA and FAM20C WT-V5 (WT), FAM20C D478A-V5 (DA), or FAM20C P328S-V5 (PS). Cell lysates were immunoprecipitated with anti-V5 antibody and the interaction was detected by WB analysis with anti-HA antibody (upper panel). Presence of FAM20A WT-HA (middle panel) and FAM20C forms (lower panel) in the same lysates was confirmed. ( C ) Binding assay by GST pull down. The rhFAM20C WT-V5/His or rhFAM20C D478A-V5/His protein was incubated with either GST protein alone or GST-FAM20A protein and coupled to glutathione beads. After washing the beads, the bound proteins were visualized by WB analysis with anti-V5 antibody (upper panel). Presence of rhFAM20C WT-V5/His (upper panel, lane 5), rhFAM20C D478A-V5/His (upper panel, lane 6), GST and GST-FAM20A (lower panels) proteins were assessed by WB analysis.

Article Snippet: An aliquot of the GST-FAM20A protein generated was subjected to WB analysis with anti-FAM20A or anti-GST (goat polyclonal, GE Healthcare Life Sciences, product code: 27-4577-01) antibody and the immunoreactivity of the protein was confirmed.

Techniques: Binding Assay, Cell Culture, Transfection, Immunoprecipitation, Western Blot, Activity Assay, Incubation

Primer sequences.

Journal: Scientific Reports

Article Title: FAM20A binds to and regulates FAM20C localization

doi: 10.1038/srep27784

Figure Lengend Snippet: Primer sequences.

Article Snippet: An aliquot of the GST-FAM20A protein generated was subjected to WB analysis with anti-FAM20A or anti-GST (goat polyclonal, GE Healthcare Life Sciences, product code: 27-4577-01) antibody and the immunoreactivity of the protein was confirmed.

Techniques: Sequencing