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Jackson Immuno
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Vector Laboratories
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BID Antibody raised in Goat validated in E, WB, IF in Human.
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Goat polyclonal to CDSN. Isotype Note: IgG Host Note: Goat Conjugation Note: Unconjugated Reactivity Note: Human Application Note: ELISA, WB
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Image Search Results
Journal: iScience
Article Title: GPR162 is a beta cell CART receptor
doi: 10.1016/j.isci.2023.108416
Figure Lengend Snippet:
Article Snippet: The following antibody combinations were used for the PLA: goat anti-GPR162 (sc-138323, dilution 1:500, Santa Cruz) with rabbit anti-CART (code 2059A, dilution 1:5000, kindly provided by Dr. Jes T. Clausen, Novo Nordisk, Måløv, Denmark), and
Techniques: Isolation, Recombinant, RNA Extraction, Enzyme-linked Immunosorbent Assay, RNA Sequencing, Software
Journal: Scientific Reports
Article Title: FAM20A binds to and regulates FAM20C localization
doi: 10.1038/srep27784
Figure Lengend Snippet: ( A ) Binding assay in a cell culture system. The 293 cells were transiently transfected with FAM20A-Flag (lane 2) and FAM20C-HA (lanes 2 and 3). Cell lysates were prepared and immunoprecipitated (IP) with anti-Flag antibody. The interaction was detected by Western blotting (WB) with anti-HA antibody (upper panel). Presence of FAM20C-HA (middle panel) and FAM20A-Flag (lower panel) in the same lysates was verified. ( B ) FAM20A-FAM20C interaction is independent from FAM20C kinase activity. The 293 cells were transfected with FAM20A WT-HA and FAM20C WT-V5 (WT), FAM20C D478A-V5 (DA), or FAM20C P328S-V5 (PS). Cell lysates were immunoprecipitated with anti-V5 antibody and the interaction was detected by WB analysis with anti-HA antibody (upper panel). Presence of FAM20A WT-HA (middle panel) and FAM20C forms (lower panel) in the same lysates was confirmed. ( C ) Binding assay by GST pull down. The rhFAM20C WT-V5/His or rhFAM20C D478A-V5/His protein was incubated with either GST protein alone or GST-FAM20A protein and coupled to glutathione beads. After washing the beads, the bound proteins were visualized by WB analysis with anti-V5 antibody (upper panel). Presence of rhFAM20C WT-V5/His (upper panel, lane 5), rhFAM20C D478A-V5/His (upper panel, lane 6), GST and GST-FAM20A (lower panels) proteins were assessed by WB analysis.
Article Snippet: An aliquot of the GST-FAM20A protein generated was subjected to WB analysis with anti-FAM20A or
Techniques: Binding Assay, Cell Culture, Transfection, Immunoprecipitation, Western Blot, Activity Assay, Incubation
Journal: Scientific Reports
Article Title: FAM20A binds to and regulates FAM20C localization
doi: 10.1038/srep27784
Figure Lengend Snippet: Primer sequences.
Article Snippet: An aliquot of the GST-FAM20A protein generated was subjected to WB analysis with anti-FAM20A or
Techniques: Sequencing